To accurately obtain the three-dimension (3D) velocities fields of the rotating flow fields, digital holography particle image velocimetry (DHPIV) is presented. A new method, namely the integrated gray-level gradient, is used to locate the focal plane of the particle and to extract the 3D particle′s coordinate. The 3D cross-correlation pairing algorithm is applied for the pairing of particles. The rotating flow fields′ 3D visual velocities fields, corresponded to the theoretical modeling, are obtained by DHPIV based on the integrated gray-level gradient method and the three-dimension cross-correlation pairing algorithm. The experimental results demonstrate that this technology can be applied for measurement of the rotating flow fields effectively.