Optical Technique, Volume. 48, Issue 3, 277(2022)
Multimodal phase imaging based on dual-view transfer of intensity equation
Living cell observation reveals the life state of cells, which is the basis of many researches in biomedicine. However, most living cells are difficult to observe because of the transparency. Zernike Phase Contrast (ZPC) and Differential Interference Contrast (DIC) are usually used to enhance the image contrast. But the commonly used ZPC and DIC microscope are complex and expensive, and rely on Wollaston prism, phase contrast ring and other specific optical components. A multimodal phase imaging method based on dual-view transfer of intensity equation is proposed. With the defocus images acquired by two cameras installed in binocular tube of the microscope, the quantitative phase of the object can be obtained by solving the transfer of intensity equation, the ZPC image and the DIC image of the object could subsequently be computed. The results of numerical simulations and imaging experiments show that the dual-view multimodal phase imaging method can provides high quality ZPC and DIC image. The proposed method doesn’t rely on specific optical components or complex configuration, and is low-cost and easy to implement. Thus, it can substitute the expensive ZPC microscope and DIC microscope in the application of unlabeled biological imaging.
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CHEN Chao, LIU Chen. Multimodal phase imaging based on dual-view transfer of intensity equation[J]. Optical Technique, 2022, 48(3): 277
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Received: Sep. 27, 2021
Accepted: --
Published Online: Jan. 20, 2023
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CSTR:32186.14.