This study introduces a method for multiparticle force measurement and rheological analysis using a high-precision optical tweezers system, aiming to address the challenge of simultaneous characterization of interaction forces and medium viscoelasticity at the micro/nanoscale. Experiments utilizing the Aresis Tweez 300 scanning optical tweezers system achieved trapping and manipulation of 2.5-μm-diameter SiO₂ particles, enabling high-resolution measurement of solvent-mediated interparticle forces (approximately 20 fN) and investigation of cellulose solution rheology (1% mass concentration), including storage modulus and viscosity. The results demonstrated that the scanning optical tweezers system could accurately measure interparticle interaction forces and characterize the dynamic response of complex fluids. By combining multicycle averaging techniques and real-time tracking methods, the signal-to-noise ratio of the system was significantly enhanced, rendering it suitable for dynamic analysis at the micro/nanoscale. This research highlights the broad application potential of scanning optical tweezers technology in colloid science, biophysics, and materials science, providing a novel methodology for micro/nanoscale force and rheology studies.