A pushbroom microscopic hyperspectral imaging device was developed to study the normal and leukemic blood cells of human. The microscopic hyperspectral images of blood from normal and leukemic human were collected and processed. Some typical transmitted spectrum curves of blood cells were extracted and analyzed. The results show that the transmittances of leukaemia blood cells are 50 percent higher than those of the normal at 541.3 nm. From the images and spectrums of the blood,it can be seen that the microscopic hyperspectral imaging device can be used to study the changes of spectrum characters and physical chemistry composition of the human blood.