The dry eye condition and ocular surface disease index were collected by questionnaire through online guidance and offline random selection of college students, so as to screen students with dry eye syndrome. By investigating the situation of dry eye and to analyze the risk factors and symptoms among college students in Shenzhen, countermeasures and suggestions for early intervention of dry eye were provided. A total of 1 200 questionnaires were obtained, of which 955 were valid, 164 students were found to have the risk of dry eye, and the prevalence rate was 17.17%. Among them, the prevalence risk rate of boys was 14.40%, and that of girls was 19.02%. There was no significant difference between male and female (χ2=3.477, P>0.05). The factors influencing dry eye among college students were contact lens wearing, refractive surgery, use of eye medicine and poor sleep quality or staying up late. Results showed that the prevalence rate of the college students was at a upper-middle level. Refractive surgery and poor sleep quality or staying up late were the most common risk factors for dry eye among the college students.