A new dinuclear manganese(Ⅱ) complex with bis-pyridine pendant-arms was synthesized by condensation between 3,3′-((ethane-1,2-diylbis((pyridin-2-ylmethyl)azanediyl)) bis(methylene))bis(2-hydroxy-5-methylbenzaldehyde) and 2-hydroxy-1,3-diaminopropane in the presence of manganese(Ⅱ). The complex was determined by X-ray diffraction single crystal structure analysis, and the corresponding formula is ［Mn2(C37H43N6O6)］·(ClO4)2. The results indicate that the complex crystallizes in monoclinic, space group P21/c, with a=1.096 50(19) nm, b=1.419 5(3) nm, c=3.109 4(5) nm, β=108.153(5)°. The crystal structure shows that the two manganese(Ⅱ) ions in the phenolbased macrocyclic dinuclear complex are coordinated with (Namine)2(Nimine)2O3 and (Nimine)2O4 sites, the corresponding geometry around each manganese(Ⅱ) center are decahedron and distorted octahedron. Two manganese(Ⅱ) centers are equivalently bridged by the phenolic oxygens and an acetate radical with the intermetallic separation of 0.331 6 nm. Moreover, the binding ability of the complex toward calf thymus DNA were analyzed by voltammetric and viscosity method, which indicate the bonding mode between them is weak intercalation.