Detect guided optical fiber′s some imperfections which are produced in the winding process with X-ray. Through the analysis of traditional X-ray image and the simulation of point X-ray scanning, the feasibility of optical fiber imperfection scanned by X-ray is confirmed theoretically, and propose that through the two-dimensional scanning of flaw to locate it, then the influence of suitable winding gap is analyzed. Finally the relevant experimental results are given, the relevant issues of the experiments are analyzed.