In order to screen out superior Auricularia auricula strains suitable for cultivation in Bijie area, the cultivation experiments of 7 strains from 3 regions and the optimization of the substrate formula were carried out, and the high-yield cultivation techniques of A. auricula in Bijie area were integrated. The results showed that A. auricula strain Qingpi No. 1 and strain B had good adaptability, high yield and were suitable for cultivation in Bijie area. The optimum temperature for rod culture was 20-25℃. The formula 100(wood chips 84%, bran 13.6%, soybean meal 1.4%, lime 0. 3%, light calcium carbonate 0.7%, moisture content 55%-60%) had the best fruiting effect. Based on the comparative test analysis of wood chip particle size, it is beneficial to increase the A. auricula yield to use the formula with fine wood chips (1-2 mm).