The aim of this study is to comprehensively explore the impact of novel fiber optic X-ray grids on imaging quality and to evaluate their potential applications in medical imaging. Using experimental analysis and comprehensive evaluation, we systematically investigated the effects of the source image distance, product of tube current and time, tube voltage, and filter grid array area on the performance of these fiber optic X-ray grids in the imaging process. Further, we comprehensively analyzed the performance of fiber optic X-ray grids by measuring the transmission of primary radiation, scattered radiation, and total radiation and image quality.Results show that under the exposure conditions of 60 kV, 40 mAs; 80 kV, 40 mAs; and 120 kV, 20 mAs, the 80-, 100-, and 120-μm novel fiber optic X-ray grids show good scattering suppression effects, which improve the quality and clarity of X-ray imaging. Additionally, the 100-μm fiber optic X-ray grids exhibit better performance in balancing the transmissions of total radiation and scattered radiation compared with the 80- and 120-μm fiber optic X-ray grids. The experimental results indicate that fiber optic X-ray grids have great potential for considerably reducing scattered radiation and improving image quality and contrast. Thus, this study offers new possibilities for diagnosis and treatment in the field of precision medicine.