Ramet propagation is the main made of propagation for Centella asiatica. In order to improve the efficiency of ramer propagation， we studied the factors that affect ramet propagation of C. asiatica. The ramet seedlings were cultivated using substrates with different ratio of vermiculite to nutrient soil. The morphology， physiology， and the content of asiaticosides were investigated. Using paraffin sections and microscopy， the structures of stolon nodes at various developmental stages were examined. The results showed that the aerial part of C. asiatica grew most vigorously and the roots grew well when the substrate with a 1∶2 ratio of vermiculite to nutrient soil was applied. In this substrate ratio group the contents of water， chlorophyll， protein， soluble sugar， and starch， as well as the contents of asiatic acid and madecassic acid were not lower than those of the other ratio groups. The structures of stolon nodes in various developmental stag?es remained unchanged and possessed the capacity to propagate. By screening stolon nodes and appropriate substrate for ramet propagation， the present work would serve as the foundation for high quality cultivation of C. asiatica.