With the continuous upgrade of display industry, display panel resolution is gradually changing from HD to FHD, which makes the quality requirements of display glass substrates more stringent. For production enterprises, it is particularly critical to improve the quality to meet market demand. In order to meet the requirements of FHD market, this study researched on particle cleaning technology for display glass substrates surface. By adjusting spray angle, disc brush, roll brush pressure and rotation speed of cleaning equipment, thus improving the quality of glass baseboard for display. Particles on the surface of display glass substrate were reduced and verified by production process, which provide improved ideas and direction for particle cleaning for display glass substrates surface.