An optical lens performance evaluation method based on multi-band regional mutual information (MRMI) is proposed. Object plane and its image plane is decomposed into multiple frequency band by wavelet transform. The retina is sensitive to different signals at different frequencies in human visual system（HVS）. So the wavelet transform simulates this process. Regional mutual information (RMI) is calculated for each sub-band according to information theory. The weighted coefficients of RMI in each sub-band are calculated by integral ratio of wavelet filter and MTF in spatial frequency domain. Then MRMI is done by weighted synthesis of RMI. Six lenses with different Abbe number and refractive index on the market were selected for measurement experiments, and the imaging MRMI value of each lens was obtained, which quantitatively reflected the imaging performance of the lens and was consistent with the visual perception of human eyes.