Taking the protein subcellular localization experiment of tobacco instantaneous transformation as an example, an experimental teaching model combined with scientific research topics is reported, which emphasizes the exploration of experimental principles and strengthens the experimental teaching process. The practice shows that this innovative approach can promote the continuous improvement and development of botany experimental teaching and cultivate high-quality talents with more practical ability and innovative thinking. It can be expected that the approach proposed can not only be applied to the field of botany, but also in other courses, play a positive role in promoting the development of experimental teaching in higher education.