Chromosome samples were prepared from dividing cells of root topa of broad bean, maize, wheat, and barley, etc. by means of aquash method. Miero-disseetion of chromosome was performed on the microscopic stage using argon laser microirradiation (514.5 nm). The chromosomes could be dissected into 12,6-9,6 and 4-6 chromosomal fractions respectively while the suitable laser power densities were applied. The establishment of laser micro-dissection of chromosome in plants provides the possibillity of the application of laser microbeam irradiation in chromosome engineering, assignment of genes, and DNA micro-cloning from chromosomal fragment in crons.