A measurement method of the spacial coordinates using camera calibration based on Genetic Algorithm (GA) and Least Square Support Vector Machine (LSSVM) was proposed for improving the traditional optical measurement method in range. First of all, the idea of camera calibration using LSSVM was presented, and its application in range measurement was discussed. The two crucial problems of choosing the training samples and confirming the samples capacity were solved by simulation analysis in range application. Finally, the measurement method of camera calibration using LSSVM was validated by two tests in range. The result shows that precision of the method is good and it has the characteristics of the simple principle, high precision, easy implementation and data processing compared with the traditional optical measurement method in range, which has important application values.