In order to further improve the microhardness of 316L substrate and improve its application fields, different weight ratios of Fe60-WC cladding layers were prepared on the surface of 316L substrate by laser cladding. Then the morphology，microhardness, and crystal phase composition of laser cladding layers with different ratios of WC were explored. According to the experimental analysis, when the weight ratio of WC is 3%, the laser cladding reveals eutectic structure with Fe60, and its microhardness is obviously increased. Furthermore, when the weight ratio of WC increased to 5%, the microstructure of the cladding layer reduces distinctly. The result shows that when the weight ratio of WC is 3%, the microhardness of the cladding layer is obviously higher than other simples.