To explore the expression of transforming growth factor-β induced gene (TGFBI) in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) and its relationship with clinical significance, Oncomine, GEPIA, LinkedOmics, Kaplan-Meier Plotter and Ualcan databases were used to analyze the expression of TGFBI in CESC, the relationship between TGFBI expression and clinical characteristics of CESC, its impact on the prognosis of patients with CESC, and the methylation of TGFBI in tumor tissue. Timer was used to analyze the relationship between TGFBI gene and tumor immune cells infiltration. The results have indicated that the expression level of TGFBI in CESC tissues was higher than that in normal cervical tissues; the methylation level of TGFBI in CESC tissue was lower than that in normal cervical tissues. There is a significant correlation between the expression of TGFBI and the clinicopathological parameters of CESC patients. The expression of TGFBI was singnificantly correlated with histological type, race, age, tumor purity, radiotherapy, T stage, N stage, M stage and the number of lymph node metastasis (P<0.05). The overall survival and progression-free survival were significantly shorter in the TGFBI overexpression group than that in the low expression group (P<0.05). The study was conducted to elucidate that TGFBI is highly expressed in CESC and is associated with the occurrence and development of CESC, which greatly increases the possibility of TGFBI as a potential therapeutic target.