The effective extraction of target analytes from complex surface is of great significance for the extension of surface enhanced Raman scattering (SERS) spectroscopy to practical applications. In this paper, a composite of the gold nanoparticles array (AuNPs) deposited on the surface of indium tin oxide conductive glass (ITO) with good SERS activity is used as a SERS substrate by using a transparent and adhesive tape. A rapid detection method for SERS measurement with pesticide residues was established. Flexible tape was used for direct extraction of the anlytes from actual fruit and vegetable surfaces and then coated onto the gold nanoparticle array (AuNPs/ITO) used as a SERS substrate for SERS spectroscopic analysis. Through a simple “paste, peel and re-paste” process, the analytes can be effectivly collected for SERS measurements, and pesticide residues from fruits and vegetables, such as thiram, can be analyzed rapidly by SERS spectroscopic measurements. This research can provide useful information for the establishment of a relevant detection technique.