Chinese Journal of Lasers, Volume. 47, Issue 5, 0509001(2020)

Elimination Method of Halo Artifacts in White-Light Diffraction Phase Microscopy

Haining Ding1,2,3, Miaomiao Zhu4, Lihong Ma1,2,3、*, and Yong Li1,2,3
Author Affiliations
  • 1College of Physics and Electronic Information Engineering, Zhejiang Normal University, Jinhua, Zhejiang 321004, China
  • 2Institute of Information Optics, Zhejiang Normal University, Jinhua, Zhejiang 321004, China
  • 3Key Laboratory of Researching Optical Information Detecting and Display Technology in Zhejiang Province, Jinhua, Zhejiang 321004, China
  • 4College of Engineering, Zhejiang Normal University, Jinhua, Zhejiang 321004, China
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    The phase image is inevitably affected by the halo effect because of the low spatial coherence of the extended white light source. Therefore, we propose a novel method of eliminating the halo effect based on the Hilbert transform in this study. This method does not require any prior knowledge about the measured sample and only needs Hilbert transform for obtaining the derivative of the measured phase image. Subsequently, the corrected low-frequency data and high-frequency data obtained from the acquired image after applying the Hilbert transform and from the original measured image, respectively, are carefully mixed. This method effectively eliminates the halo effects and preserves the high resolution of the white-light phase imaging system. The phase images obtained via white-light diffraction phase microscopy, including those of the standard polystyrene microspheres and the living red blood cells, are processed by applying the proposed method. The experimental results demonstrate that the proposed method can effectively and rapidly eliminate the halo effects associated with the white-light diffraction phase image.

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    Haining Ding, Miaomiao Zhu, Lihong Ma, Yong Li. Elimination Method of Halo Artifacts in White-Light Diffraction Phase Microscopy[J]. Chinese Journal of Lasers, 2020, 47(5): 0509001

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    Paper Information

    Category: holography and information processing

    Received: Nov. 3, 2019

    Accepted: Dec. 19, 2019

    Published Online: May. 12, 2020

    The Author Email: Ma Lihong (zjnumlh@zjnu.cn)

    DOI:10.3788/CJL202047.0509001

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