By using the laser cladding technique, three kinds of Ni-Mo-Si coatings are prepared on copper substrate surfaces. The phase compositions and microstructures of these coatings are researched, and the hardness and toughness of these coatings are studied with the indentation method. The study results show that the phase compositions of three kinds of coatings are MoSi2+Ni2Si+γ-Ni (coating 1, Ni-20Mo-40Si), Mo5Si3+Ni2Si+γ-Ni (coating 2, Ni-30Mo-30Si), and Mo2Ni3Si+Moss+γ-Ni(coating 3, Ni-40Mo-20Si), respectively. The average hardness of coating 1 is 1100 HV and its average friction coefficient is 0.44. The average hardness of coating 2 is 1200 HV and its average friction coefficient is 0.50, but its toughness of fracture is poor. The average hardness of coating 3 is 860 HV, its average friction coefficient is 0.55, and its toughness of fracture is good. The wear track depths for coatings 1-3 are 6.30, 5.26 and 7.00 μm, respectively.