In order to study effect of fluorescence decay of ethyl acetate in the simulated liquor environment, the steady-state and time-resolved fluorescence spectra of ethyl acetate ethanol aqueous solution with different volume fractions were measured. Combined with quantum chemical calculation, experimental measurements and theoretical analysis were carried out. The results show that, for ethyl and acetate, there are two fluorescence peaks at 407nm and 431nm, respectively. Fluorescence lifetime is about 1.4ns. There are two fluorescence lifetimes in the decay process of ethyl acetate ethanol aqueous solution with different volume fractions. The short-life component is ethyl acetate. The long-life component is polymer structure formed by ethyl acetate, ethanol and water molecules. Intermolecular interaction in the structure of the polymer improves the planarity of ethyl acetate. This is conducive to radiation transition. Water molecules connect ethyl acetate and ethanol through hydrogen bonds and van der Waals forces to form layered structures, block the movement of the fluorescent body, reduce non-radiative transitions, and prolong fluorescence lifetime. This result is helpful to enrich the detection methods of organic compounds in liquor. It is also helpful to study the changes of molecular conformation and spectral characteristics in solvent environment.