The sonic wave method is a non-destructive testing method for measuring the modulus of glass materials, but it is limited by the shape and size of samples. Limitations can affect the accuracy of testing the modulus of glass fibers using the sonic wave method due to factors such as the transmission efficiency of the transmitted signal, sensitivity of reception, and differences between samples. This study explored the feasibility of using the acoustic wave method to test the modulus of glass fibers by adjusting the frequency of the acoustic vibration and the number of fibers in the yarn. The results show that with an increase in frequency (5 kHz), the signal intensity through the glass fiber decreases, while vibrations at 200 Hz exhibit good accuracy (coefficient of variation ≤ 3.73%) and goodness of fit (R2=0.999 9). Furthermore, compared with the tensile method, it was found that the test results were consistent, the number of fibers in the yarn increased, the elastic modulus decreased, and the modulus of elasticity of single filaments was higher than that of multifilaments. It provided a reference for future non-destructive testing of fiber material modulus.