To solve the problem of automatic selection the segmentation scale in remote sensing image, a superpixel segmentation method of high resolution remote sensing image based on hierarchical clustering is proposed. Firstly, the watershed segmentation algorithm based on adaptive morphological reconstruction is used to segment the image into multiple superpixels. Then, the gray feature vectors of each superpixel is extracted. Finally, the hierarchical clustering method is adopted to merge the superpixels, the accurate segmentation of high-resolution remote sensing images is realized. Four sets of remote sensing images are selected in the experiment, and the experimental results are evaluated by a combination of qualitative and quantitative methods. Experimental results shown that the proposed method effectively improves the segmentation accuracy of remote sensing images, and better segmentation visual effects are obtained.