Prehnite can be produced in the form of platy, schistose, botryoidalis, kidney, radial or massive aggregates. In recent years, prehnite has attracted the attention of many scholars due to its beautiful appearance and special crystal structure. In this paper, the composition, structure and spectral characteristics of yellow-green prehnite were investigated by the electron microprobe, powder X-ray diffractometer, fourier transform infrared spectrometer, micro-Raman spectrometer, and ultraviolet-visible spectrophotometer. The main chromogenic element of prehnite is Fe. Fe3+ often replaces Al3+ to occupy the octahedral coordination position, and Fe2+ often replaces Ca2+ into the lattice channel. The electron microprobe results show that the content changes of Fe and Al are negatively correlated as a whole, and the content changes of Fe and Ca are also negatively correlated as a whole. The samples with relatively high Fe content have a deeper yellow-green tone. XRD patterns and Raman spectra show that epidote exists in the form of inclusion in prehnite. Infrared and Raman spectra show that there are two framework of silicon-oxygen tetrahedron and aluminum-oxygen octahedron in prehnite. The UV-Vis absorption spectra reveal the chromogenic mechanism of prehnite. This paper systematically analyzes the mineralogical and spectral characteristics of prehnite, so as to provide ideas and experimental data for the further research of prehnite.