Utilizing high energy lasers for transmission line tree barrier removal has shown promising outcomes. This paper investigates the influencing factors on laser clearing efficiency for four tree species, focusing on laser power, tree species, water content, and wind speed. The results show that the ablation efficiency is negatively correlated with tree density and positively correlated with water content, wind speed, and laser power. The higher the density of trees burned through, the lower the efficiency and the longer it takes to clear the obstacles. When the trees with 50.4% moisture content are burned by laser, it is not easy to produce a carbonization layer that hinders the penetration of laser energy. The removal time is shorter than the trees with 14.2% moisture content. With the same power laser, the barrier removal efficiency of the line laser is much higher than that of the point laser. When the diameter of trees is 10.50 mm~35.50 mm, the time required to use a point laser is 2.48~6.12 times that of a line laser. With the increase in diameter, the point laser takes more time to remove, indicating that the larger the diameter of the tree barrier, the higher the efficiency of using the line laser. This research result provides an essential basis for applying and developing laser tree barrier removal devices.