Referring to limitation of the conventional mercury lamp spectral calibration in ultraviolet wave band, a spectral calibration device was built for ultraviolet spectroradiometer. The physical process and measurement chain of spectral calibration were studied and twelve ozone absorptive spectral lines were calibrated. The measurement uncertainty and combined standard uncertainty of influenced quantities of wavelength calibration were analyzed and the combined standard uncertainty was 0.026nm. Spectral bandwidths can reach 1.0nm according to the analysis of optical CAD and experiment results. The spectral calibration system does well in ultraviolet spectral calibration for spectroradiometer. The calibrated results satisfy the spectral precision for ozone retrieval.