In this paper, the original avocado oil and normalized Raman spectra of avocado oil, palm oil, corn oil, mustard seed oil, walnut oil and flax seed oil were compared by a portable laser Raman spectrometer. The results showed that: avocado oil has two characteristic Raman peaks with weak and unique peaks at 1115 cm-1 and 1523 cm-1, and the unsaturation parameter value I1296/I1260 of avocado oil is much higher than that of 5 other oils. Raman spectroscopy was also used to detect 21 kinds of avocado oil actual samples. It was found that there was one sample Raman spectroscopy and that there were big differences in characteristic peak intensity and unsaturation parameters, such as I1296/I1260, which may be incorporated into other cheap oils. Fatty acid composition test was used to further verify that the sample was adulterated. Experimental results found that: the portable laser Raman spectrometer could be used for the rapid identification of avocado oil.