In order to improve the wear resistance of the ball valve, the laser cladding is used to construct the strengthening layer of the biological structure imitating the leaf pattern on the surface of the stainless steel, and the microstructure, hardness, and wear resistance of the sample are tested and analyzed. The results show that the microstructure is rapidly cooled, the grain is refined during the laser cladding process, and the surface hardness of the cladding layer is greatly improved, forming the "vein" structure in the bionics. However, due to multiple overlapping and secondary scanning, the grains continue to grow and the hardness decreases, forming the "mesophyll" structure in biomimetic. The "vein" and "mesophyll" are staggered, with the higher hardness being "vein" and the lower hardness being "mesophyll". "Leaf vein" plays a supporting role in the skeleton, and "mesophyll" plays a buffering role in filling the skeleton. Among the different coupling bionic paths, the specimen with an angle of 60° between the laser scanning path and the friction and wear direction has the best wear resistance. The oblique bionic structure is beneficial to release the stress in the friction pair and reduce the fatigue wear phenomenon. The main wear mechanism is a small amount of abrasive wear and adhesive wear.