Glass fibers with different diameters and cylindricity were prepared by mechanical wire drawing process, and the upper and lower limits of glass annealing temperature were tested by fiber weight-bearing method, and the effects of fiber geometric parameters, load weight quality and temperature control conditions on the upper and lower limits of annealing temperature test results were investigated. The results show that the upper and lower limits of annealing temperature increase with the increase of glass fiber diameter and decreases as weight mass increases. With the increase of cylindricity, the temperature of the upper and lower limits of annealing decreases. According to the verification results, the suitable conditions for testing the upper and lower limit temperature of glass annealing are that the fiber diameter of 0.6～0.8 mm, the load weight of 800～1 000 g, the heating rate of 5 ℃/min, and the cooling rate of 4 ℃/min, and at the same time, taking into account the safety of the test, the initial temperature is recommended to choose a lower temperature for the experiment.