At present, liquid crystal with fused heterocyclic ring is an important direction of molecular structure design and development. In this paper, a new liquid crystal compound with 5,6-difluorobenzofuran ring structure was synthesized via palladium catalyzed coupling reaction and ring closing reaction of cyclohexyl substituted acetylene and o-iodophenol derivative. The total yield was 54%. Its structure was characterized by 1H NMR and MS, and its phase transition temperatures were tested by DSC and POM. It is found that the melting point and the clear point of the compound is 118 ℃ and 202 ℃ respectively, and the nematic temperature range is 84 ℃. The physical properties test shows that the birefringence is about 0.13, the dielectric anisotropy is 12.6 and the rotational viscosity is 420 mPa·s. Compared with the similar 3,4-difluorobenzene liquid crystal compound, the birefringence of the compound containing 5,6-difluorobenzofuran ring increases by 65%, the dielectric constant increases by 97%, and the clear point increases by 66%. As a new kind of fluorine-containing building block, 5,6-difluorobenzofuran has outstanding comprehensive performance and good application prospect in the field of liquid crystal display.