Two spherical transducers (SP32K and SP12K) are developed for the marine sediment acoustic in-situ measurement system, which are comparable to the ITC-1007 and ITC-1032 spherical transducer. The transducer is simulated and designed by theory and finite element method, and the transducer prototype is fabricated and its performance is tested. The resonant frequency of the low frequency transducer is about 12 kHz, with the operating frequency band covering from 2 to 20 kHz, and the maximum transmission voltage response is 149 dB. The resonance frequency of the medium frequency spherical transducer is about 32 kHz, with the working frequency band covering from 15 to 50 kHz, and the maximum transmission voltage response is 149 dB. The experimental results show that compared with similar foreign products, the developed spherical transducers have basically the same performance, which can meet the relevant acoustic measurement requirements, and provide a strong support for the development of acoustic equipment such as medium and low frequency marine sediment acoustic in-situ measurement systems.