The long growing period of cotton in the Yangtze river basin is not conducive to the planting of cotton oil in the double cropping system. By regulating cultivation factors, the cotton growing period can be shortened, so as to realize cotton oil single cropping and improve the planting efficiency.Early maturing cotton variety “JX0010” was used in this research as test material. The effects of nitrogen concentration,density and planting period on the accumulation and distribution of cotton dry matter were studied by using the quadratic optimal regression design(311 design). According to regression model analysis; and according to the single factor effect analysis of each cultivation factor and seed cotton yield, the order of its effects on seed cotton yield is as follows:sowing date>planting density>nitrogen application. The seeding period has obvious negative effect on seed cotton yield, and nitrogen fertilizer and density have obvious positive effect on seed cotton yield. The order in which the two factors interact on the yield of seed cotton is as follows:the interaction effectsof nitrogen and planting density>density and seeding period>Nitrogen application and sowing date. Therefore, in production, we should make full use of the interaction effect of nitrogen fertilization and density, pay attention to the reasonable combination of the two, and combine the planting period to promote the increase of cotton production.