To investigate the influence degree of temperature and humidity regulation in the standard environment on the physical indicators of cigarettes. Taking 21 component cigarettes of 16 cigarette brands including regular cigarettes, medium cigarettes and thin cigarettes as the research objects, 16 indicators such as cigarette weight, hardness, opening resistance and ventilation rate were measured before and after the adjustment of environmental temperature and humidity. Paired two-sample mean analysis and correlation analysis were conducted on the test data. The results showed that after the temperature and humidity adjustment, the moisture content of the cigarette samples increased by 0.924 percentage points, the filling value of the tobacco shreds decreased by 0.206 cm³/g, the hardness of the cigarettes decreased by 4.078 percentage points, and the indentation amount of the hardness test increased by 0.267 mm. This might be due to the increase in moisture absorption of the tobacco shreds during the adjustment process, which in turn led to a decline in the filling performance of the tobacco shreds. There were no significant differences in the weight of cigarette auxiliary materials, open suction resistance, closed suction resistance, paper ventilation, nozzle ventilation, and total ventilation before and after temperature and humidity adjustment. However, there were significant differences in cigarette weight, net filament content, moisture content, filament filling value, filling density, hardness, indentation diameter, pre-pressing diameter, and indentation amount. The correlation coefficient R² between the pre-pressing diameters of cigarette samples before and after adjustment was 0.994 62, and the correlation coefficient R² between the indentation amounts was 0.918 97, both reaching above 0.90, with a strong correlation. Through the relevant mathematical model, the hardness of cigarette samples after adjustment can be quickly predicted.