The bubble structure of glass lenses had been studied by middle infrared (MIR) spectroscopy. The infrared absorption modes of bubble structure of glass lenses mainly include νasO=C=O-one dimension. The thermal stability of bubble structure of glass lenses was further studied by temperature varying MIR spectroscopy. It was found that the main functional groups (νH-O-H-one dimension、νasO=C=O-one dimension and δO=C=O-one dimension) absorption frequency and intensity of the bubble structure of glass lenses had changes in the temperature range of 303～393 K. The main functional groups (νH-O-H-two dimension, νasO=C=O-two dimension, δH-O-H-two dimension, νsO=C=O-two dimension and δO=C=O-two dimension) information about the sensitivity to heat and the change speed of bubble structure of glass lenses were further studied by two-dimensional MIR spectroscopy. It is found that the infrared absorption modes of glass lens bubble structure are mainly concentrated in 3 420～3 380 cm-1, 2 365～2 335 cm-1, 1 660～1 630 cm-1, 1 350～1 330 cm-1 and 680～660 cm-1,about 5 frequency ranges, which show different spectral performance. This paper had expanded the research scope of three-stage MIR spectrum (including MIR spectrum, variable temperature MIR spectrum and two-dimensional MIR spectrum) in the bubble structure and thermal denaturation of important optical products (glass lenses).