3D laser scanning technology is an active remote sensing technology with stable, fast and precise performance, and it can scan thousands of points to a point cloud of 3D data of the object and be operated at night. 3D laser scanning technology is used to make an inventory of warehouse, takes the horizontal bin of Beijing Qingyundian grain storage and the squat silo of Zhuozhou grain storage of China grain reserves corporation for examples, emphasizes the method of its application on this aspect, including the processing of situ measurement of 3D laser scanner, data processing and volume calculating. The results of the experiments show that the application of 3D laser scanning technology on surveying of grain volume has great practicality for its high accuracy and fast speed.