In this paper, Tm 3+ doped and Ho 3+/ Yb 3+ co-doped tellurite-germanate glasses were prepared by the high-temperature melting method, and their mid-infrared fluorescence performances at 2 μm were studied. The results showed that as for the Tm 3+ doped tellurite-germanate glass pumped by the 808 nm laser diode, the full width at half maxium of its fluorescence at 1.81 μm was 211 nm and the corresponding emission cross-section was 6.32×10 -21 cm 2. In contrast, when the Ho 3+/ Yb 3+ co-doped tellurite-germanate glass pumped by the 980 nm laser diode, the widest fluorescence full width at half maximum of its fluorescence at 2.03 μm was 170 nm and the corresponding emission cross-section was 3.2×10 -21 cm 2. The research results demonstrate that the rare earth ions doped tellurite-germanate glass has not only the excellent physicochemical properties, but also the excellent fluorescence performances at 2 μm, which is one of the most promising gain materials for fiber lasers in the mid-infrared wavelength range.