Laser bird repelling has promising applications in agriculture, power, and aviation safety with its excellent performance. To safely and efficiently expel birds, the mechanism of laser interaction with the internal tissues of the bird′s eye needs to be studied. Firstly, the Pennes biological heat transfer equation and the Beer-Lambert equation are used to establish a three-dimensional dynamic model to simulate the absorption and transmission of laser light in the bird′s eye tissue. Secondly, the steady-state simulation is used to analyze the temperature change of the bird′s eye under different conditions, and the steady-state results are used as the initial value of the transient study to improve the accuracy of the simulation results. Finally, a laser bird-repelling experimental platform was established and experimental studies were conducted. The results showed that the temperature of the anterior part of the bird′s eyeball was greatly affected by the ambient temperature and tear evaporation, while the temperature of the posterior part was relatively stable. During laser irradiation, the highest temperature was observed on the retina, with variations of up to 13 ℃ among different tissue regions. When using a light spot diameter of 3.5 mm, a laser power ranging from 1.5 mW to 2.7 mW, and an irradiation duration of 0.4 s, the maximum temperature could be maintained between 52 ℃ and 60 ℃. Simulation and experimental validation confirmed that the determined laser parameters could effectively disperse birds without causing any harm to their eyes.