This report presented a detailed study on parameters and mechanisms of cleaning rubber layer from a tyre wording mould with pulse laser. The results demonstrated that pulse laser can remove rubber layer completely under proper parameters. There exist cleaning threshold and damage threshold in laser cleaning. Between the initial cleaning threshold and complete cleaning threshold, cleaning percentage of the surface increases with the increasing of laser fluence. When the laser fluences are between complete cleaning threshold and damage threshold, cleaning percentage keeps up 100% and no damage is found on substrate surface. Two mechanisms for laser cleaning rubber layer are suggested: one is the combustion and vaporation of upper rubber layer resulted by high temperature of laser beam, the other is the vibration of deep rubber layer as well as thermal shock action by laser pulse to make rubber particles sputter.