The intensity profile of the non-diffracting Bessel beams disturbed by an opaque obstacle was studied. The two-dimonsional optical intensities distribution in difference propagation distances was simulated numerically. Second time reconstruction of the Bessel beam was demonstrated, and the relative parameters were measured. The minimum reconstruction distances are 44 mm and 60 mm respectively when the obstacle dimensions are 400 μm and 520 μm. It shows that the smaller the dimension of the obstacle is, the easier the beam reconstruction. The simulation and experimental results show that the obstructed Bessel beam can reconstruct itself in free space, even achieve multi-plane . This result has an important practical significance in multi-plane micro-manipulation.