Optical coherence tomography (OCT) is a high resolution, contact-free and non-invasive optical imaging approach. By measuring the interference signal of the backscattering light of the tissues, OCT can obtain the interior structure of the tissues. As a diagnostic technique, OCT has been widely used in medical examination, especially in ophthalmological imaging diagnosis. Frequency-domain OCT (FD-OCT) has a better performance in the imaging speed than that of time-domain OCT (TD-OCT), as it can obtain the characteristic information of the whole depth without mechanical scanning. Therefore, it is more applicable to ophthalmology. We constructed a fiber based FD-OCT imaging system, of which the axial resolution is less than 10 μm and scanning speed is more than 24000 times per second. The system can image the retinal tissue structure for clinical diagnosis.