Electroporation is a physical process that transiently permeabilizes prokaryotic and eukaryotic cell membranes with an electrical pulse, permitting cell uptake of a variety of biological molecules. Because of the advantages of high efficiency, low toxicity popularity, and controllability, it has been applied in the fields such as cell engineering, genetic engineering, and so on. The principles and characteristics of gene transfection apparatus which is made by us will be illustrated in this paper. The temperature influence is considered and the optical fiber sensor in the apparatus is proposed.