The application and testing mechanism of the two common activation energies of glass melt,namely conductivity activation energy and viscosity activation energy, were introduced. On this basis, the conductivity activation energy and viscosity activation energy of the glass melt with typical E glass formula were calculated. The results showed that the conductivity activation energy was 162.6 to 194.9 kJ/mol,and the viscosity activation energy was 247.3 to 257.2 kJ/mol. After B2O replaced CaO with equal mass fraction, the electrical resistivity and viscosity of glass melt increased, but the conductivity activation energy and viscosity activation energy of the glass melt decreased. It is shown that the activation energy of glass melt is affected by the change of components, and the specific mechanism and degree of influence need to be further studied.