Lhasa as the core area of the national ecological security barrier construction faces the risk of bio-ecological security caused by the invasion of Datura stramonium In order to explore the impact of the invasion of Datura stramonium on soil seed bank characteristics in Lhasa this study took the soil seed bank of Datura stramonium with different invasion levels within the urban area of Lhasa central Tibet Plateau as the research object and revealed the differences in the soil seed bank of soil with different invasion pressures by means of field surveys and seed germination experiments aiming at providing a basis for the scientific prevention and control of the invasive plants in Tibet The results show that 1 The total number of plants in the soil seed bank was 46 belonging to 18 families and 43 genera mainly annual herbs Among them there were 10 invasive alien species which w distributed in each sample site 2 Mild invasion of Datura stramonium significantly increased the species diversity of plant communities in the invaded site P<0 05 but the community diversity tended to decrease with deeper invasion 3 Pearson correlation analyses showed that both light and heavy invasive pressure from Datura stramonium had negative effects on the native species Digitaria cruciata Lepidium apetalum Artemisia wellbyi Plantago depressa and Pseudognaphalium affine This study clarifies the effect of Datura stramonium invasion on the community structure of soil seed bank in Lhasa area and provides some scientific basis for the Datura stramonium of local biological invasions