OBJECTIVE To investigate the effect of multiband-light-emitting diode (LED) in regulating cutaneous cells’ functions after skin injury. METHOD The epidermal keratinocytes (HaCaT) and dermal fibroblasts (HS27) of human were injured by mechanical injury, respectively. The injured cells were treated by a LED light that with four peak bands (460 nm, 590 nm, 640 nm, and 940 nm) and output energy density of 400 mW/cm2. The proliferation and migration of HaCaT cells were evaluated by CTG assay and wound healing assay, respectively. Moreover, the proinflammatory factors in HS27, such as interlukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were detected by ELISA. In addition, the extracellular matrix (ECM) proteins production of HS27 were evaluated by western blotting and ELISA. RESULTS Low level light therapy (LLLT) with the multiband-LED i) accelerated cell proliferation and migration of injured keratinocytes, ii) decreased the protein levels of IL-6 and TNF-α in the injured fibroblasts, iii) increased the production of Collagen-I, Collagen-III, and hyaluronic acid. Furthermore, a better improved wound healing effect were seen after two treatments, compared with that after only one treatment. CONCLUSION The multiband-LED mediated photobiomodulation accelerated re-epithelialization, ameliorated dermal inflammation and stimulated ECM production, promoting wound healing of skin.