In order to solve the problems of low efficiency and poor accuracy of traditional small size LCD panel defect addressing method applied to high resolution and large size LCD panel, a full automatic accurate defect addressing system was established, and its hardware and software architecture, implementation and defect addressing logic were studied. Firstly, an addressing central control system was constructed by adding a movable optical camera mechanism and corresponding software architecture on the basis of detection equipment. Then, the system can accurately detect the defect by comparing the pictures taken by camera before and after the hollow cross cursor displayed by the LCD panel coincided with defect. Finally, the defect coordinates were output through the LCD panel driving device. After five types of defects test, the results show that the system is stable and easy to use, as well as fully automatic, faster recognition and 100% accurate addressing. After the application of the system in the defect coordinates addressing process of H company, the accuracy of defect coordinates increases by more than 30%, which brings significant improvement in repair benefits. The system has great application value in the field of defect coordinates addressing of large size LCD panel.