A method was established for simultaneous determination of ten active components in Qingkailing injection (QKL), and could be used as a feasible method for the quality control of QKL. HPLC-DAD-ELSD was employed for the determination at the optimal wavelength (255 nm). Fifteen samples of QKL at different batches from different factories were analyzed by the means. The results show that the method is corresponding with specified standard. The contents from different samples (QKL from different factories and different batches) have great disparity (The RSD values range from 2.17% to 65.54%). It is concluded that the method is reliable and convenient for the quality control of QKL. The key point of QKL quality control is to control the chief activity components as much as possible.