Natural biological enzymes have the advantages of high catalytic activity and selectivity, while they are usually sensitive to the matrix and extreme environmental conditions. Nano-enzymes with higher stability can be effective substitutes and supplements for biological enzymes in catalytic reactions. Enzyme catalysis and Fenton-like reactions have shown great potential in environmental pollutant degradation. Metal-organic frameworks (MOFs) are excellent carriers for biological enzymes and other functional molecules. The in-situ precipitation method prepared ZIF-8 composites of hemin and glucose oxidase (Hemin@GOx@ZIF-8). Hemin@GOx@ZIF-8 has good dispersibility in an aqueous solution, and the solution appears dark green. The structure characterization showed that Hemin and GOx could be successfully encapsulated in the ZIF-8framework, and the skeleton structure of ZIF-8 remained intact. Hemin@GOx@ZIF-8 has a porous structure with an average particle size of 300 nm, a specific surface area of 1 512 m²·g^-1, an average aperture of 1.076 nm, and a pore volume of about 0.607 5 cm³·g^-1. Large specific surface area and pore volume can provide more active sites for catalytic reactions, which improves catalytic efficiency. Hemin@GOx@ZIF-8 has good stability and cascade catalytic performance. Under glucose drive, H₂O₂ is generated from reactions catalyzed by GOx, then catalyzed by Hemin to complete the Fenton-like reaction. Thus, organic pollutants are efficiently catalyzed and degraded in this cascade catalytic system. The catalytic degradation performance of the cascade system was studied using the organic dye Congo Red (CR) as the substrate. The results indicated that the degradation rate of CR in aqueous solution reached over 88%. The cascade catalytic system has good thermal stability, organic solvent resistance, and repeatability. Further research indicated that Hemin@GOx @ZIF-8 has good degradation ability for some different organic pollutants in water. So this cascade catalytic system has important practical significance and good application prospects in environmental pollutant degradation.