Efficient cutting of float glass can be achieved by using 532 nm nanosecond laser with the bottom-up spiral scanning method. Due to the obvious thermal effect when the nanosecond laser acts on the glass, it will cause glass edge chipping, white spots on the side wall surface, surface micro cracks and voids and other defect behaviors. Focusing on this problem, the single-factor experimental method was used to explore 532 nm laser hole cutting defect behavior. The results show that the laser pulse frequency is the key factor affecting the defect shape. Improper pulse frequency and pulse energy will seriously increase the glass edge chipping and the surface roughness of hole wall, and also produce uneven regional white spots. Increasing the scanning speed will reduce the surface roughness of hole wall. Smaller spiral overlap rate will cause the corrugated surface morphology of glass hole wall. When the hole wall quality is poor, the micro cracks and voids are more obvious.