The role of recombinant collagen in improving wrinkles, enhancing skin elasticity, firmness and improving skin complexion was explored. 32 qualified subjects were selected and enrolled in the lactate stinging test group. After using the samples continuously for 28 days, the maximum depth and width of crow’s feet, skin tightness (F4), melanin index, erythema index, and red-green chroma (a^*) value all showed significant reductions (P<0.01). Additionally, skin roughness (P_a) significantly decreased (P<0.05), while skin elasticity (R2), skin brightness (L^*), and individual type angle (ITA°) values all significantly increased (P<0.01). Thus, type III recombinant collagen has the effects of anti-wrinkle, firming, soothing and improving skin complexion.