It is always been the difficulty to measure flow rate and phase content ratio of oil-water two-phase flow for the petroleum development. In this paper, the distributed acoustic sensing (DAS) technology is proposed for the acquisition and transmission of oil-water two-phase flow vibration signals of pipe wall. Axial stretching of the winding fiber consists two components: turbulent eddies vibration and sound speed of the turbulent flow. Then the mixing flow rate and phase content ratio are calculated by the fiber phase amplitude. To improve the sensitivity of vibration signal measurement with fiber, a winding fiber sensing model is proposed. We analyze the effects of two parameters, winding pitch and the number of tightly winding turns, on the vibration amplitude of the pipe wall and the sound speed along the axial direction of winding fiber, and experimentally verify three of the winding methods. It turns to more accurately measure the vibration of the pipe wall and the sound speed of the fluid with the winding method of 4 tightly winding turns at a pitch of 0.02 m. The R2 of fitting curves between the standard deviation of the fiber phase amplitude and the flow velosity within the pipe reaches 94%, and the calculated relationship between sound speed and oil content ratio is fully consistent with the theory.